FIG. 12.

(A) Infectiousness of the BH10 wild-type virus and the BH-T24N, as well as BH-V13G-T24N mutated viruses in MT-2 cells. (B) Viral genomic RNA levels in the wild-type and mutated viruses determined by RT-PCR using primer pair pGAG1-pST. RNA samples of each virus were diluted 1:2 and 1:4 to ensure the linear range of the reactions. Wild-type viral DNA standards of 10¹, 10², 10³, and 10⁴ copies were used to determine the linear range of reactions. Lane 1, viral RNA from viruses equivalent to 200 pg of CA Ag; lane 2, 100 pg of CA Ag; lane 3, 50 pg of CA Ag. Intensity of RNA signals was quantified using the NIH Image program with the levels of wild-type BH10 arbitrarily set at 1.0.