FIG. 7.

Susceptibility of HeLa (CAR positive) and RD (CAR negative) cells to Ad5/FibWT virus (dark columns) and Ad5/FibR7-RGD recombinant (light columns), as determined by the level of expression of reporter gene GFP. Cells were infected with an equal multiplicity of infection (10 PFU/cell) for 1 h at 37°C. The efficiency of Ad5-mediated gene transfer was estimated by counting the GFP-positive cells by FACS analysis at 24 h postinfection, and results are expressed as the percentage of positive, fluorescent cells (mean of three separate experiments ± SD).