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. 1990 Nov 1;271(3):785–790. doi: 10.1042/bj2710785

Expression of epidermal-growth-factor receptor in the K562 cell line by transfection. Altered receptor biochemistry.

H Allen 1, J Hsuan 1, S Clark 1, R Maziarz 1, M D Waterfield 1, R A Flavell 1, J Haley 1
PMCID: PMC1149632  PMID: 2173908

Abstract

The epidermal-growth-factor (EGF) receptor was expressed in the human erythroleukaemic cell line K562 by transfection of the receptor cDNA. EGF-receptor biochemistry appears altered in the K562 transfectants. Autophosphorylation of the K562 receptor is not stimulated substantially by EGF. Tyrosine kinase activity of the receptor is high in the absence of EGF, whereas receptor affinity for EGF is low. K562 cells are shown to lack mRNA for transforming growth factor alpha (TGF alpha). Therefore autocrine stimulation of the K562 receptor, at least by TGF alpha, does not explain the observed receptor biochemistry. The K562 receptor is phosphorylated at a single major site in intact cells, a threonine residue that may be Thr-669. Possible mechanisms of regulation of the EGF receptor in the K562 transfectants are discussed.

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