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. 2024 Oct 22;15:9107. doi: 10.1038/s41467-024-53486-w

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Macroscopic images of native bovine fat tissue (left) and solely dynamically cultured and differentiated fat spheroids after harvest (right). Scale bar: 3 cm and 1 cm; b Overview of saturated (SFA), monounsaturated (MUFA) and polyunsaturated (PUFA) fatty acids in differentiated (Diff) and control (Ctrl) spheroids and bovine fat tissue (Fat).; c Fatty acid composition in detail. Significances are noteable for SFA for Fat vs. Diff (p = 0.0077) and Diff vs. Ctrl (p = 0.0052). MUFA: Diff vs. Ctrl (p = 0.0009); d Fatty acid spectrum of all samples.; e Most abundant fatty acids. Significances are noteable for C18:0 for Fat vs. Ctrl (p < 0.0001) and Diff vs. Ctrl (p < 0.0001). C18:1cis: Fat vs. Diff (p = 0.0017), Fat vs. Ctrl (p = 0.0393), Diff vs. Ctrl (p < 0.0001).; All data are plotted as mean and for (c–e) ± SD. Dots represent single data points.; Significances were calculated using two-way analysis of variance and Holm-Šídák multiple comparisons test; *: p ≤ 0.05, **: p ≤ 0.01, ***p ≤ 0.001; n = 3 biological replicates. Source data are provided as a Source Data file.