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. 2024 Oct 22;14:24266. doi: 10.1038/s41598-024-73955-y

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Degradation and assimilation of glycosaminoglycans (GAGs) and mucin by C. perfringens strain ATCC 13124. (a,b) Halo assay for the degradation of GAGs (HA, CSC, and HP). Plates before (left) and after (right) addition of acetic acid in nutrient-poor (a) and nutrient-rich medium (HA, glucose-rich medium; CSC and HP, GAM medium) (b). c growth of C. perfringens cells in the presence of HA or mucin. Circle, nutrient-poor medium; triangle, nutrient-poor medium containing HA; and square, nutrient-poor medium containing mucin. Each measurement represents the mean of three individual experiments. Significant differences from the control (nutrient-poor medium) were determined using Student’s t-test (**p < 0.01, *p < 0.05).