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. 2024 Oct 22;14:24266. doi: 10.1038/s41598-024-73955-y

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Hyaluronan-degrading activity of C. perfringens strain ATCC 13124. Native-PAGE followed by CBB staining (a) and halo-forming activity staining (Plates before (left) and after (right) addition of acetic acid. The yellow frame indicates the native-PAGE gel.) (b), and TLC (c). CpeHysA, the purified recombinant CpeHysA; C. perfringens S, the culture supernatant of C. perfringens; C. perfringens C, the cell extract of C. perfringens; C. perfringens C (x40), 40-fold concentrated C. perfringens C. HA, hyaluronan; ∆HA, unsaturated HA disaccharide.