Fig. 3.

Exogenous expression of Rp downregulates endogenous Rp levels by proteasomal degradation

(A–D) Wing discs harboring endogenously expressed EGFP-RpS20. In the wing pouch region, RpS20-HA is exogenously expressed (B), and Rpt2 (C) or Rpn1 (D) is simultaneously knocked down. (E) Quantification of the ratio of EGFP intensity in the wing pouch region to other regions. Black dots show individual measurements and gray bars show the average. ***p<0.001; Steel-Dwass test. (F–I) Wing discs harboring endogenously expressed EGFP-RpL5. In the wing pouch region, RpL5-HA is exogenously expressed (G), and Rpt2 (H) or Rpn1 (I) is simultaneously knocked down. (J) Quantification of the ratio of EGFP intensity in the wing pouch region to other regions. Black dots show individual measurements and gray bars show the average. ***p<0.001; Steel-Dwass test.