FIG. 2.

Presentation of detoxified CyaAs carrying multiple epitopes to anti-LCMV LC3A10 and anti-OVA B3Z class I-restricted T-cell hybridomas. APCs (splenocytes from BALB/c [A] or C57BL/6 mice [B]) were incubated in the presence of various concentrations of the CyaA toxoids, harboring either OVA (●) or LCMV (□) epitope at site 224 or the three epitopes (MEP) at different sites (108 [■], 233 [▴], or 336 [▾]) and were cocultured, respectively, with the anti-LCMV CD8⁺ hybridoma LC3A10 (A) or the anti-OVA CD8⁺ hybridoma B3Z (B). IL-2 secretion by the stimulated hybridoma was determined by the CTL proliferation assay. Results are expressed in cpm of incorporated [³H]thymidine (cpm in the presence of CyaA − cpm in the absence of CyaA). Data represent the mean values of duplicate samples (standard error of the mean, <10%) and are representative of two (A) and three (B) experiments.