FIG. 6.
Immunization with the detoxified CyaA toxoids carrying multiple epitopes induces full protection against a lethal LCMV challenge. On days 0 and 14, BALB/c mice were immunized with 50 μg of wild-type CyaA E5 (mock control, CyaA wt-E5) or the recombinant CyaA carrying the LCMV epitope alone (CyaA224LCMV-E5) or the polyepitope at different positions (CyaA108MEP-E5, CyaA233MEP-E5, or CyaA336MEP-E5), mixed with 1 mg of alum. Control groups received either PBS or 105 foci of LCMV i.p. On day 21, the mice were challenged intracerebrally with 101.7 foci of LCMV. Mortality was monitored for 21 days. The percentage of mice that survived and the number of surviving mice out of the total number of challenged mice are shown for each group. The data represent the cumulative results of two experiments.