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. 2024 Dec 1;24(6):1606–1619. doi: 10.17305/bb.2024.10328

Figure 4.

Figure 4.

Overexpression of ASCL1 inhibits the proliferation, invasion, and migration of HCC cells. (A and B) qRT-PCR and WB detected the expression of ASCL1 in normal and HCC cell lines; (C and D) qRT-PCR and WB detected the overexpression efficiency of ASCL1 in HCC cell lines (SNU-387 and MHCC-97H); (E and F) CCK-8 detected cell proliferation in HCC cell lines (SNU-387 and MHCC-97H) overexpressing ASCL1; (G and H) Transwell assay detected cell invasion and migration of overexpressed ASCL1 in HCC cell lines (SNU-387 and MHCC-97H). The scale bar is 50 µm. *P < 0.05, ***P < 0.001. HCC: Hepatocellular carcinoma; WB: Western blotting; CCK-8: Cell-counting kit-8; qRT-PCR: Quantitative real-time polymerase chain reaction; ASCL1: Achaete-scute family bHLH transcription factor 1.