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. 2024 Dec 1;24(6):1606–1619. doi: 10.17305/bb.2024.10328

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© 2024 Zhang et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure 6. — Overexpression of SLC6A13 increases ethanol-induced inflammatory cytokine production. (A and B) CCK-8 detection on the viability of HCC cell lines (SNU-387 and MHCC-97H) after ethanol treatment at different concentrations (0, 5, 15, 25, 40, 50 mM) for 24 h; (C and D) qRT-PCR detected the effect of overexpression of SCL6A13 on the expression of inflammatory factors (IL-1β, IL-6, and TNF-α) in HCC cell lines (SNU-387 and MHCC-97H) under 50 mM ethanol treatment; (E and F) ELISA experiment detected the effect of overexpression of SCL6A13 on the secretion levels of inflammatory factors (IL-1β, IL-6, and TNF-α) in HCC cell lines (SNU-387 and MHCC-97H) under 50 mM ethanol treatment. *P < 0.05. CCK-8: Cell-counting kit-8; HCC: Hepatocellular carcinoma; ELISA: Enzyme-linked immunosorbent assay; SLC6A13: Solute carrier family 6 member 13; qRT-PCR: Quantitative real-time polymerase chain reaction; IL-1β: Interleukin-1 beta; IL-6: Interleukin-6; TNF-α: Tumor necrosis factor-alpha.