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. 2024 Dec 1;24(6):1606–1619. doi: 10.17305/bb.2024.10328

Figure 7.

Figure 7.

Glycine promotes inflammatory resistance of SLC6A13 overexpression to ethanol treatment. (A and B) CCK-8 detected the effect of different concentrations of exogenous glycine on the cell viability of SNU-387 and MHCC-97H cells; (C and D) ELISA experiment detected the expression of amino acid metabolism-related genes after control and over-SLC6A13 in SNU-387 and MHCC-97H cells; (E and F) qRT-PCR detection of inflammatory factors (IL-1β, IL-6, and TNF-α) expression in HCC cell lines (SNU-387 and MHCC-97H) after adding 50-mM ethanol for 24 h in three groups: Control and over-SLC6A13, over-SLC6A13+Gly (8 mM); (G) WB detection of inflammatory factors (IL-1β, IL-6, and TNF-α) expression in HCC cell lines (SNU-387 and MHCC-97H) after adding 50 mM ethanol for 24 h in three groups: Control and over-SLC6A13, over-SLC6A13+Gly (8 mM). *P < 0.05, #P < 0.05, ***P < 0.001. CCK-8: Cell-counting kit-8; ELISA: Enzyme-linked immunosorbent assay; WB: Western blotting; HCC: Hepatocellular carcinoma; SLC6A13: Solute carrier family 6 member 13; qRT-PCR: Quantitative real-time polymerase chain reaction; IL-1β: Interleukin-1 beta; IL-6: Interleukin-6; TNF-α: Tumor necrosis factor-alpha.