Table 1.
Target | Direction | Sequence |
---|---|---|
SLC6A13 | Forward | 5′-GGGCATTGACAGCCAGTTCT-3′ |
SLC6A13 | Reverse | 5′-AAGTCTGGGGTACTCGTCCA-3′ |
ASCL1 | Forward | 5′-GAAGCAGGATGGCAGCAGAT-3′ |
ASCL1 | Reverse | 5′-TCGGGCTTAGGTTCAGACAC-3′ |
IL-1β | Forward | 5′-ATGAAAGACGGCACACCCAC-3′ |
IL-1β | Reverse | 5′-ATGAAAGACGGCACACCCAC-3′ |
IL-6 | Forward | 5′-GACAAAGCCAGAGTCCTTCAGA-3′ |
IL-6 | Reverse | 5′-TGTGACTCCAGCTTATCTCTTGG-3′ |
TNF-α | Forward | 5′-AGGCACTCCCCCAAAAGAT-3′ |
TNF-α | Reverse | 5′-TGAGGGTCTGGGCCATAGAA-3′ |
GAPDH | Forward | 5′-TTGCCCTCAACGACCACTTT-3′ |
GAPDH | Reverse | 5′-TGGTCCAGGGGTCTTACTCC-3′ |
qRT-PCR: Quantitative real-time polymerase chain reaction; ASCL1: Achaete-scute family bHLH transcription factor 1; IL-1β: Interleukin-1 beta; IL-6: Interleukin-6; TNF-α: Tumor necrosis factor-alpha; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.