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Purification of VP4. (A) Gel filtration chromatography. Chromatogram of VP4 separated on a Hi-Load 16/60 Superdex 200 gel filtration column as a final purification step. The Vo is 44.1 ml. Solid line, A280; dashed line, calibration curve; circles, elution volumes of MW markers. (B) SDS-PAGE. Coomassie blue-stained SDS-polyacrylamide gel of purified recombinant VP4. Lane MW, molecular mass standards labeled in kilodaltons; lane 4, VP4.
