Figure 2.

(A) Potency of 7G5.TCR-T against MR1-expressing NSCLC line A549 or A549 MR1 knockout cells. ELISpot assays were used to measure IFN-γ secretion by 7G5.TCR-T cells in response to the targets at the indicated effector-to-target ratios. Representative IFN-γ ELISpot showing reactivity of 7G5.TCR-T to MR1 wild-type or MR1 knockout A549 cells. The data shown in (A) is representative of at least six biological replicates. ^**** p < 0.0001 statistical difference between reactivity seen with 7G5.TCR-T against A549WT cells versus A549.MR1KO, by one-way ANOVA with Tukey’s multiple comparison test. (B) MR1 expression on cancer cell lines as compared to isotype control, plotted against 7G5.TCR-T reactivity data from one donor (IFNy data expression is normalized to a response from nontransduced T cells). Data are shown as IFN-γ production from T cells following co-culture with cancer cell lines. Each point is an individual cancer cell line. Grey circles are MR1*04 cell lines, and black circles are non-MR1*04. There is no significant correlation between IFN-γ production and MR1 expression datasets as computed using nonparametric Spearman’s correlation and a two-tailed t-test (p = 0.58).