FIG. 2.

Western blotting of K562 lysate with K5.M MAbs. K562 cells were lysed in TKMT at 37°C for 1 h. Clarified lysate was run on a 4 to 20% gradient acrylamide SDS-PAGE gel under nonreducing and reducing conditions and subjected to Western blot analysis as described in Materials and Methods with the MAbs indicated.