FIG. 8.

BCD does not alter VCAM-1 binding. K562/VCAM cells were pretreated with BCD, followed by preincubation with anti-VCAM-1 MAb VIII6G10 or media alone. They were then incubated with Jurkat T cells for 1 h at 37°C as described in Materials and Methods. Conjugate formation was visualized by photomicroscopy 1 h after mixing the cells. Top row, left to right: untreated Jurkat cells and untreated K562/VCAM1 cells alone, mixed with Jurkat cells, and mixed with Jurkat cells in the presence of anti-VCAM1 MAb (with anti-VCAM1). Bottom row, left to right: BCD-treated K562/VCAM1 cells alone, mixed with Jurkat cells (middle panel), and mixed with Jurkat cells in the presence of anti-VCAM1 MAb (right panel).