Figure 2.

TMEM16F prevents the PM leakage from Lm‐elicited injury. A) Quantification of PS exposure (left) and cell death (right) of thymocytes treated with the low dose of ionomycin (1 µM) upon Lm infection (n = 6). B) Representative histogram of ZsGreen fluorescence in thymocytes isolated from TMEM16F‐ZsGreen reporter mice (fluorescent intensity arbitrarily defined as low, intermediate, and high). C, D) Representative histogram of Annexin V (C) and PI (D) staining in thymocytes population with low, intermediate, and high level of ZsGreen after Lm infection (left) and the correlation analysis between ZsGreen and Annexin V or PI (right) (n = 12 for C and D). All the experiments were repeated for at least twice independently. Data are presented as mean ± SEM. The “n” represents the technical replicates. Two‐way ANOVA for A, and Spearman correlation analysis for C and D. ^* p < 0.05, ^** p < 0.01, ^*** p < 0.001.