Figure 5.

Cytopathological evaluation in the cell rotation process. a.I–III) Definition of three commonly used nuclear morphology parameters: solidity, circularity, and N/C ratio. b) Eight representative observation angle of a single hematoxylin‐stained Huh7 cell. c) Quantification of the three nuclear parameters for the Huh7 cell group (n = 30). d) PCA analysis of the nuclear morphology variation under different observation angles for the Huh7 cells (n = 30). All p‐values were determined using one‐way ANOVA (normally distributed) or Kruskal‐Wallis tests (non‐normally distributed). NS: no significance. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Scale bar, 10 µm.