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Cloning and purification strategy for influenza rHA expression. (A) Amino acid sequence of recombinant ectodomain A/Astrakhan/3212/2020 HA (left) and full‐length HA (right). The leader sequence is shown in green, the trimerization sequence in blue, and the His‐tag sequence in red. (B) Schematic diagram of the purification scheme used for Expi293 expressed recombinant ectodomain and full‐length influenza HA (created with BioRender.com).
