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Characterization of Expi293 expressed H5 rHA. (A) Recombinant A/gyrfalcon/Washington and A/Astrakhan H5 HA from transfected Expi293 cells was purified and characterized by SDS‐PAGE under reducing (r) and non‐reducing (nr) conditions. Gels were stained with Coomassie blue (left) or transferred to nitrocellulose membranes for analysis by Western blot (right). (B) Purified rHA was analyzed for HA activity using chicken red blood cells and a starting protein concentration of 0.05 mg/mL.
