Fig. (4).

Transient Transfection of MCF-7, MTT Assay for Cell viability, and Invasion Assay of PHB. (a) Green fluorescence protein intensity among groups (OE, NC, and MOCK group ordinally). Cells were imaged with a fluorescence microscope (200×). Relative mRNA expression of PHB. GAPDH was used as an internal reference gene. (b) Representative western blot image of PHB and β-actin gene and densitometric analysis. (c) Cell viability was measured by MTT assay at 24, 48, or 72 hours following transfection of MCF-7 with pEGFP-PHB plasmid. (d) Representative transmembrane cell image of three groups after staining with crystal violet, and its statistical analysis of the total number of transmembrane cells in the three groups from 10 random fields. (e) the invasive ability of MCF-7 cells was measured by Transwell Ma trigel assay. *p < 0.001 or p < 0.05 versus NC control; Abbreviations: GAPDH=Glyceraldehyde 3-phosphate Dehydrogenase; OE=Overexpressed Group; NC=Negative Control Group.