Shikonin alleviates the imbalance of Th1/Th2 and Th17/Treg in OVA- and HDM-induced asthma mice. Flow cytometry was used to evaluate the percentages of CD4+ IFN-γ+ Th1 cells (a), CD25+ FOXP3+ Treg cells (b), CD4+ IL-4+ Th2 cells (c), and CD4+ IL-17+ Th17 cells (d) in lung tissues. Compared with the control group, **
p < 0.01; compared with the OVA group, ##
p < 0.01; compared with the HDM group, △△
p < 0.01, n = 3.