Table A.1.
Primer sequences and PCR conditions
| Gene | Origin | Strand | Sequence | Size (bp) |
ATa (°C) |
|---|---|---|---|---|---|
| Mmp3 | Mouse |
bS cAs |
5'-CTGTGTGTGGTTGTGTGCTCATCCTAC-3' 5'-GGCAAATCCGGTGTATAATTCACAATC-3' |
350 | 60 |
| Mmp13 | Mouse | S As |
5'-TGATGGACCTTCTGGTCTTCTGGC-3' 5'-CATCCACATGGTTGGGAAGTTCTG-3' |
473 | 60 |
| Cox-2 | Mouse | S As |
5'-GGTCTGGTGCCTGGTCTGATGAT-3' 5'-GTCCTTTCAAGGAGAATGGTGC-3' |
724 | 63 |
| Col2a1 | Mouse | S As |
5'-CACACTGGTAAGTGGGGCAAGA-3' 5'-GGATTGTGTTGTTTCAGGGTTCG-3' |
173 | 58 |
| Acan | Mouse | S As |
5'-CCTGCTACTTCATCGACCC-3' 5'-AGATGCTGTTGACTCGAACCT-3' |
150 | 60 |
| Sox9 | Mouse | S As |
5'-GAGCCGGATCTGAAGAGGGA-3' 5'- GCTTGACGTGTGGCTTGTTC-3' |
151 | 58 |
| Gapdh | Mouse | S As |
5'-TCACTGCCACCCAGAAGAC-3' 5'-TGTAGGCCATGAGGTCCAC-3' |
450 | 60 |
a
AT, annealing temperature.
b
S, sense.
c
As, antisense.