Table 2.
mRNA and Protein Expression a
| HEAT | SHAM | |||||
|---|---|---|---|---|---|---|
| PRE-IMMO | POST-IMMO | RTS | PRE-IMMO | POST-IMMO | RTS | |
| mRNA expression (A.U.) | ||||||
| HSP70 | 27.2 ± 1.1 | 27.4 ± 1.2 | 27.6 ± 1.1 | 27.0 ± 1.4 | 28.0 ± 2.1 | 26.7 ± 1.5 |
| HSP90 | 23.0 ± 0.6 | 23.2 ± 0.6 | 23.3 ± 0.6 | 23.1 ± 0.9 | 23.1 ± 0.8 | 22.6 ± 0.7 |
| MuRF-1 | 27.5 ± 2 | 28.1 ± 1.8 | 28.4 ± 1.6 | 27.8 ± 1.9 | 28.4 ± 1.6 | 27.1 ± 1.5 |
| MAFbx/atrogin-1 | 0.9 ± 0.6 | 0.5 ± 0.2 | 0.6 ± 0.3 | 1.2 ± 0.4 | 0.6 ± 0.4 | 0.8 ± 0.5 |
| Protein expression (A.U.) | ||||||
| p-AKT | 14.9 ± 7.8 | 18.7 ± 5.6 | 16.3 ± 5.5 | 12.2 ± 5.1 | 23.1 ± 10.5 | 18.6 ± 8.5 |
| AKT total | 28.6 ± 13.8 | 31.7 ± 15.9 | 29.3 ± 9.4 | 24.2 ± 13.4 | 29.9 ± 14.6 | 28.5 ± 14.8 |
| p-NFκB | 12.1 ± 5.3 | 14.6 ± 5.2 | 12.2 ± 4.5 | 12.0 ± 4.9 | 20.2 ± 9.0 | 15.3 ± 8.8 |
| NFκB total | 7.4 ± 3.7 | 11.5 ± 6.0 | 9.6 ± 4.7 | 9.1 ± 3.8 | 12.9 ± 5.1 | 10.3 ± 3.9 |
| p-FoxO | 46.7 ± 27.9 b | 49.4 ± 25.4 b | 63.9 ± 27.3 b | 48.4 ± 35. 1 | 55.8 ± 35.6 | 64.0 ± 43.7 |
| FoxO1 total | 39.3 ± 23.1 | 36.4 ± 20.0 | 36.4 ± 32.4 | 41.4 ± 28.0 | 51.8 ± 32.5 | 55.6 ± 51.8 |
a
The caspase 3 and phosphorylated/total ratios are presented in Figure 5. AKT, protein kinase B; A.U., arbitrary units; FoxO, fork head box O proteins; HEAT, heat therapy group; HSP, heat shock protein; MAFbx/atrogin-1, muscle atrophy F-box; MuRF-1, muscle RING-finger protein-1; NFκB, nuclear factor-kappa B; p-, phosphorylated; POST-IMMO, postimmobilization; PRE-IMMO, preimmobilization; RTS, Return To Sport (i.e. test after rehabilitation); SHAM, control group. Data in mean ±SD.
b
P < .05, effect of time in HEAT.