FIG. 3.

In vitro-translated DHBV TP and RT-RH domains interact posttranslationally in a Dɛ-dependent manner. The indicated constructs were separately expressed in RRL, and translation was stopped by adding cycloheximide to a final concentration of 20 μg/ml. The TP- and RT-containing lysates were mixed and incubated together with Dɛ RNA (only lane 3; final concentration, 1 μM) for 1 h at 30° C. The samples were subjected to priming assays in the presence of [α-³²P]dATP and analyzed by SDS-PAGE on the same gel as those in Fig. 2C; the exposure shown here is four times longer. See the legend to Fig. 2A for details about constructs.