TABLE 1.

Defined set of useful reagents described in this study

Reagent	Typea	Key features
pEVS104	Plasmid	Conjugal helper plasmid that increases conjugation efficiency, does not replicate in recipient cells (37)
pTNS++ (pJMP8045)	Plasmid	pBBR1 origin replicating vector expressing Tn7 transposase genes, increases Tn7 transconjugant recovery in R. sphaeroides
pJMP8602	Plasmid	Tn7 plasmid containing a promoterless mScarlet-I gene for testing promoter activity
PC	Promoter	Synthetic, IPTG-inducible promoter in R. sphaeroides and N. aromaticivorans
PD	Promoter	Synthetic, IPTG-inducible promoter in R. sphaeroides and N. aromaticivorans
PG	Promoter	Synthetic, IPTG-ducible promoter in R. sphaeroides and N. aromaticivorans
PO	Promoter	Synthetic, IPTG-inducible promoter in R. sphaeroides and N. aromaticivorans
PP	Promoter	Synthetic, IPTG-inducible promoter in R. sphaeroides and N. aromaticivorans
pJMP8921	Plasmid	Mobile-CRISPRi plasmid containing mScarlet-I reporter, PD-sgRNA (BsaI/no spacer), and PC-dCas9 for testing system (no guide control)
pJMP8823	Plasmid	Mobile-CRISPRi plasmid containing mScarlet-I reporter, PD-mScar1 sgRNA, and PC-dCas9 for testing system (mScarlet-I targeting guide)
pJMP8930	Plasmid	Mobile-CRISPRi plasmid containing PD-sgRNA (BsaI/no spacer) and PC-dCas9 for cloning new sgRNA spacers
pJMP8923	Plasmid	Mobile-CRISPRi plasmid containing mScarlet-I reporter, PD-sgRNA (BsaI/no spacer), and PG-dCas9 for testing system (no guide control)
pJMP8835	Plasmid	Mobile-CRISPRi plasmid containing mScarlet-I reporter, PD-mScar1 sgRNA, and PG-dCas9 for testing system (mScarlet-I targeting guide)
pJMP8932	Plasmid	Mobile-CRISPRi plasmid containing PD-sgRNA (BsaI/no spacer) and PG-dCas9 for cloning new sgRNA spacers

^a See Fig. 2B and Table S15 for promoter sequences.