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. 2001 Aug;75(16):7462–7469. doi: 10.1128/JVI.75.16.7462-7469.2001

TABLE 3.

Median fold changes in IC50 against viral isolates containing mutations associated with reduced in vitro susceptibility to lopinavir

Mutation Isolates containing mutation
Isolates lacking mutation
Pa by:
n Fold IC50
n Fold IC50
Median Mean Median Mean Wilcoxon ANOVA
L10F/I/R/V 69 4.6 14.5 43 0.9 1.4 <0.0001 <0.0001
K20M/R 14 30.5 29.3 98 1.6 6.6 0.0001 <0.0001
L24I 10 14.0 26.7 102 1.7 7.8 0.0011 0.0008
M46I/L 41 4.2 15.8 71 1.1 5.8 0.0003 0.0002
F53L 13 4.6 24.6 99 1.6 7.5 0.0019 0.0014
I54L/T/V 38 16.0 23.7 74 1.1 2.1 <0.0001 <0.0001
L63P 88 2.5 11.5 24 1.1 2.1 0.0026 0.0023
A71I/L/V/RT 65 3.6 11.2 47 1.1 7.1 0.0002 0.0001
V82A/F/T 40 14.0 21.5 72 1.1 2.8 <0.0001 <0.0001
I84V 19 6.1 14.8 93 1.4 8.4 0.0007 0.0004
L90M 48 3.4 12.4 64 1.1 7.3 0.0004 0.0003
a

Since the M97-765 (Virco) and M98-957 (ViroLogic) baseline isolates were analyzed using different single-cycle phenotypic assays, a sensitivity analysis that introduced a twofold discordance between the assays was performed. A twofold discordance in one direction did not affect which mutations were statistically significant using the Wilcoxon rank sum test and produced only a minor change in the outcome of the confirmatory analysis (ANOVA), wherein the L63P mutation changed from statistically to marginally significant. A twofold discordance in the other direction did not affect the statistical significance of any of the 11 mutations but produced an apparent statistically significant association of the G73A/S/T mutation. 

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