Figure 2.

Baseline barrier function of alcohol use disorder (AUD) and non-AUD airway cells. A: transepithelial resistance (TER) values of all samples immediately prior to infection. Non-AUD 1 had a lower TER compared with AUD 1 (**P = 0.007) and AUD 2 (*P = 0.04) and AUD 3 (*P = 0.01). n = 3 biological replicates per condition consisting of five Transwells per group. Values represent means ± SD in each case. B and C: cells were incubated with a mixture of calcein (B) and 10 kDa Texas Red Dextran (C) over a 2-h period and the rate of flux from the apical to basolateral surface was determined. B: the amount of calcein flux was higher for AUD 3 vs. AUD 2 (*P = 0.02) and vs. non-AUD 3 (*P = 0.02). C: the amount of 10 kDa Texas Red Dextran flux was higher for AUD 3 than non-AUD 3 (*P = 0.03). n = 3 biological replicates per condition consisting of four Transwells per group. Samples were analyzed using one-way ANOVA with Tukey multiple comparisons test.