Figure 3.

Alcohol use disorder (AUD) cells have impaired barrier function following severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Differentiated bronchial cells from subjects with AUD (A–C, red) and non-AUD (D–F, blue) were infected with SARS-CoV-2. Transepithelial resistance (TER) was measured immediately prior to infection (t = 0) and at 6, 24, 48, and 72 h postinfection, normalized to values at t = 0. AUD cells showed decreased barrier function 72 h after infection (A, *P = 0.04; B, #P = 0.11; C, ****P < 0.0001). By contrast, non-AUD cells showed increased barrier function 72 h after infection (D, *P = 0.034; E, *P = 0.046; F, ***P = 0.0002). n = 3 biological replicates consisting of five Transwells per group. Values represent means ± SD in each case. Samples were analyzed using one-way ANOVA with Fisher’s least significant difference (LSD) test.