Figure 4.

Virus shedding from infected cells. Alcohol use disorder (AUD) and non-AUD cells were infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) at multiplicity of infection (MOI) 0.1 for 6 h. Medium was collected from apical surface washes over a 72-h period and total plaque forming units (PFU) were determined using Vero E6 cells as described in methods. Non-AUD 2 cells showed the highest levels of virus shedding, which was higher than the amount of virus shed from AUD cells (*P = 0.04; #P = 0.06) n = 3 biological replicates consisting of 2–5 Transwells per group. Samples were analyzed using one-way ANOVA with Tukey multiple comparisons test. Basolateral medium did not contain detectable levels of SARS-CoV-2.