Fig. 3.

Bicistronic expression vector used to coexpress a series of TrkA/TrkB chimeric receptors (TrkA/TrkB fusion proteins) and GFP. a TrkA/TrkB chimeric receptors (A/B-wild, A/B-Shc (−), A/B-PLC (−), and A/B-KN) fuse the extracellular domain (EC) of TrkA with the transmembrane (TM) and intracellular (IC) domains of TrkB. Y504F, Y805F, and K560N are the mutations in the binding sites for these adaptor proteins. The K560N mutation gives rise to a kinase-negative receptor (A/B-KN). b Structure of the bicistronic expression vector (pBudCE4/GFP) for co-expression of GFP and TrkA/TrkB chimeric receptors. A CMV promoter was used to drive GFP expression and an EF1-alpha promoter was used to drive TrkA/TrkB chimeric receptor expression