Fig. 5.

Distribution of TrkA/TrkB chimeric receptors on the cell surface. Using a Sindbis virus bicistronic expression system, the indicated TrkA/TrkB chimeric receptor variants were co-expressed with GFP in hippocampal neurons grown in culture for 4 days. Three days later, cells were fixed for staining with antibody specific for the EC domain of the TrkA receptor. To examine the localization of the TrkA/TrkB receptors on the cell surface, cells were not permeabilized. Microscopic images were taken as described in “Materials and Methods” section. Note that all TrkA/TrkB chimeric receptors were expressed on the cell surface. Scale bar, 20 μm