FIG. 1.

E1B 19K inhibits formation of the TNF-α-induced 500-kDa Bax protein complex during adenovirus infection. HeLa cells were mock, Ad5dl309, or Ad5dl337 infected for 24 h and then untreated or treated with TNF/CHX for 4 h. Cell lysates prepared in CHAPS lysis buffer were fractionated on a Sephacryl S-300 gel filtration column. Column fractions 20 to 43 were analyzed by SDS-PAGE followed by Western blotting for Bax or E1B 19K, as indicated. Whole-cell lysates from both the minus- and plus-TNF/CHX samples loaded on the column were included on every Western blot as an internal reference for Bax or E1B 19K levels. The peaks at which the molecular weight markers fractionated are indicated.