Table 1. Examination of transparency of spot formed by ORF93 and its mutants against various bacteria using a double-layered agar plate assay.

Tested bacteria		ORF93	ORF93-Δ05	ORF93-Δ10	ORF93-Δ15
Species	Strains
S. aureus	SA27	+	+	++	++
S. schleiferi	D91	+	+	++	++
S. felis	C3	+	+	++	++
S. sciuri	D108	+	+	++	++
S. arlettae	D119	+	+	++	++
S. pettenkoferi	C58	+	+	+	++
S. kloosii	D187	+	+	+	+
S. pseudintermedius	D19	±	±	+	+
S. epidermidis	GTC289	±	±	±	+
B. subtilis	168	-	-	-	-
E. coli	JM109	-	-	-	-

The degree of transparency of the spot was examined at a 4-level scale. The levels of transparency on the spot are ranked at “strong,” “moderate,” and “weak” as “++”, “+”, and “±“, respectively; no transparency of spot is shown as “-”.