FIG. 6.

Colocalization of B5R protein with LAMP2 in cells expressing F13L-GFP. HeLa cells were transfected with pB5R alone or cotransfected with pF13L-GFP and pB5R for 24 h. One set of cotransfected cells was treated with BFA for 30 min. Cells were fixed, stained, and examined as described in the legend to Fig. 5. Unmerged and merged images are labeled with the name of a single protein or the names of two proteins, respectively. First row: green, FITC; red, rhodamine; yellow, overlap of green and red. Other rows: green, GFP; red, rhodamine; white, indodicarbocyanine (Cy5); yellow, overlap of green and red; purple, overlap of green and white; blue, overlap of white and red.