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Effect of amino acid substitutions in the phospholipase active-site motif of F13L-GFP on intracellular localization. HeLa cells were transfected with pF13L(K314R)-GFP or pF13L(D319E)-GFP or cotransfected with those plasmids and pB5R. After 24 h, the cells were fixed, permeabilized, and stained with mouse anti-p115 MAb followed by rhodamine-conjugated anti-mouse Ig antibody or rat anti-B5R MAb followed by rhodamine-conjugated anti-rat Ig antibody.
