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Effect of amino acid substitutions of the palmitylation sites of F13L-GFP on intracellular localization. HeLa cells were transfected with pF13L(C185S)-GFP or pF13L(C186S)-GFP or cotransfected with those plasmids and pB5R for 24 h. Cells were fixed, permeabilized, stained, and examined by confocal microscopy as described for Fig. 8.
