Table 1.
Amino acid sequences of the human peptides that were used for raising the granin region-specific antibodies
| Antibody directed to amino acid sequence | Corresponding peptide |
|---|---|
| CgA | |
| LK2H10 250–284a | N-terminal pancreastatin |
| 1–17 | N-terminal vasostatins |
| 17–38 | Disulfide bridge part of vasostatins |
| 63–76 | C-terminal vasostatin-I |
| 100–113 | C-terminal vasostatin-II |
| 116–130 | N-terminal chromostatin |
| 176–195 | Chromacin |
| 238–247 | |
| 284–301 | C-terminal pancreastatin |
| 324–337 | WE-14 |
| 361–372 | C-terminal catestatin (N-terminal parastatin) |
| 375–384 | Mid-parastatin, GE-25 |
| 411–424 | C-terminal parastatin |
| CgB | |
| 1–16 | |
| 16–37 | |
| 153–167 | |
| 244–255 | |
| 259–270 | |
| 312–331 | |
| 406–417 | |
| 420–432 | N-terminal GAWK |
| 439–451 | C-terminal GAWK |
| 506–517 | |
| 542–552 | |
| 555–565 | C-terminal PE-11 |
| 568–577 | N-terminal chrombacin |
| 580–593 | Mid-chrombacin, BAM-1745 |
| 622–632 | C-terminal chrombacin, mid-CCB, secretolytin (614–626) |
| 647–657 | C-terminal CCB |
| SgII | |
| 154–165 | N-terminal secretoneurin |
| 172–186 | C-terminal secretoneurin |
| 223–249 | Part of C-terminal EM-66 |
| SgIII | |
| 141–156 | |
| 348–361 | |
All antibodies were raised in rabbits, with the exception of the mouse monoclonal antibody, clone LK2H10 (Boehringer-Mannheim; Mannheim, Germany). Cg chromogranin, Sg secretogranin
aPortela-Gomes and Stridsberg (2001)