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. 2024 Oct 10;12:1491740. doi: 10.3389/fcell.2024.1491740

FIGURE 2.

FIGURE 2

Kaempferol promotes injury repair in the intestinal and enhances healthspan under injury conditions. (A) Model of Kaempferol feeding in Drosophila during BLM treatment. (B) Immunofluorescence images of Drosophila midguts fed with 25 μg/mL BLM, with or without the addition of 20 µM Kaempferol. Nuclei are stained with DAPI (blue), GFP highlights ISCs and progenitor cells (green), and Dl identifies ISCs (red). The top images show merged images, the middle images highlight ISCs and progenitor cells, and the bottom images focus on ISCs. (C) The proportion of esg-GFP+ cells to DAPI+ cells per ROI in midguts treated with BLM, with or without Kaempferol supplementation. (D) The proportion of DI+ cells to DAPI+ cells per ROI in midguts treated with BLM, with or without Kaempferol supplementation. (E) The number of pH3+ cells per fly gut, with or without 20 µM Kaempferol, under PQ/BLM treatment conditions. (F, G) Survival rates of female wild-type flies treated with DMSO (blue curve) or Kaempferol (red curve) under 20 mM PQ (F) or 5 μg/mL BLM (G) conditions. Data from three independent experiments are shown.