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. 2024 Oct 10;12:1491740. doi: 10.3389/fcell.2024.1491740

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Copyright © 2024 Zhao, Luo, Zhang, Fan, Zhang and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Kaempferol prevents ISC hyperproliferation through its antioxidative functions during aging. (A) RT-qPCR utilized to measure the mRNA levels of ROS-related genes (sod1, sod2 and Cat) in the midguts of 40-day-old wild-type Drosophila, comparing those with and without Kaempferol treatment (administered starting at 26 days). Three independent experiments were completed. (B) Representative images of DHE staining are provided. esg-GFP⁺ cells (green) and DHE staining (red) are highlighted by white dashed lines. The top images show merged images, while the bottom images focus on DHE staining. DHE fluorescence intensity was measured in esg-GFP⁺ cells, with each dot representing a single cell. (C) Quantitation of DHE fluorescence intensity in esg-GFP⁺ cells from this experiment. (D) Kaempferol further inhibited ISC proliferation in Uas-LacZ, Cat-overexpressed, and Keap1-depleted aged ISCs (starting from day 26). The top images show merged images, and the bottom images highlight esg⁺ cells, labeled with GFP. (E) The proportion of esg-GFP⁺ cells to DAPI⁺ cells per ROI in experiment (C). (F) The number of pH3⁺ cells per gut in experiment (C).