Table 6.

Physicochemical characteristics of the developed vaccine

Characteristics	Result
Number of amino acids	523
Molecular weight	56,054.94 da
Theoretical pI	9.55
Total number of negatively charged residues (Asp + Glu)	57
Total number of positively charged residues (Arg + Lys)	82
Chemical formula	C2465H3916N712O769S7
Total number of atoms	7869
Estimated half-life (mammalian reticulocytes, in vitro)	1 h
Estimated half-life (yeast, in vivo)	30 min
Estimated half-life (Escherichia coli, in vivo)	> 10 h
Instability index	26.91
Aliphatic index	60.46
Grand average of hydropathicity (GRAVY)	-0.803