Fig. 4. Validation of the 31P-MRS editing sequence.

To test and validate the 31P-MRS editing sequence, we performed the measurement during normal perfusion of the muscle and during ischemia. Here, we show changes from baseline to ischemic state of NADH contents (A), NAD⁺ content (B), and ratios of NAD⁺/NADH (C) in the calf muscle of eight healthy young volunteers (n = 8). Changes were statistically tested using a two-sided paired student t-test. A p = 0.037, B p = 0.004, C p = 0.004. ** p < 0.01, * p < 0.05.