Table 3.
Associations of miR-20a-5p expression levels with breast cancer risk factors.
| Stroma | Cytoplasm | Nucleus | Cytoplasm and nucleus | ||||||
|---|---|---|---|---|---|---|---|---|---|
| High | Low | High | Low | High | Low | High/high | Low/low | Mixed | |
| Age at diagnosis, y, median (IQR) | 52 (14) | 57 (9) | 54 (13) | 57 (8) | 56 (13) | 57 (7) | 54 (16) | 57 (7) | 57 (11) |
| p | 0.009* | 0.049* | 0.49 | 0.15 | |||||
| BMI, n (%) | |||||||||
| < 25 | 31 (68.9) | 149 (58.0) | 46 (63.0) | 135 (58.2) | 116 (65.5) | 65 (50.8) | 45 (63.4) | 64 (50.8) | 72 (66.7) |
| 25–30 | 12 (26.7) | 82 (31.9) | 22 (30.1) | 73 (31.5) | 46 (26.0) | 49 (38.3) | 21 (29.6) | 48 (38.1) | 26 (24.1) |
| > 30 | 2 (4.4) | 26 (10.1) | 5 (6.0) | 24 (10.3) | 15 (8.5) | 14 (10.9) | 5 (7.0) | 14 (11.8) | 10 (9.3) |
| p | 0.30 | 0.62 | 0.033* | 0.12 | |||||
| Age at menarche, y, mean (SD) | 12.9 (1.1) | 13.5 (1.4) | 13.1 (1.3) | 13.0 (1.3) | 13.0 (1.3) | 13.1 (1.3) | 13.0 (1.3) | 13.1 (1.3) | 12.9 (1.3) |
| p | 0.26 | 0.60 | 0.19 | 0.99 | |||||
| Menopausal status**, n (%) | |||||||||
| Post | 23 (50.0) | 194 (73.8) | 43 (58.9) | 176 (73.6) | 119 (66.1) | 100 (75.8) | 41 (57.8) | 98 (75.4) | 80 (72.1) |
| Pre | 23 (50.0) | 69 (26.2) | 30 (41.1) | 63 (26.4) | 61 (33.9) | 32 (24.2) | 30 (42.3) | 32 (24.6) | 31 (27.9) |
| p | 0.001* | 0.016* | 0.07 | 0.028* | |||||
| Parity, n (%) | |||||||||
| Parous | 42 (91.3) | 236 (89.7) | 68 (93.2) | 212 (88.7) | 160 (88.9) | 120 (90.9) | 66 (93.0) | 118 (90.8) | 96 (86.5) |
| Nulliparous | 4 (8.7) | 27 (10.3) | 5 (6.9) | 27 (11.3) | 20 (11.1) | 12 (9.1) | 5 (7.0) | 12 (9.2) | 15 (13.5) |
| p | 0.74 | 0.27 | 0.56 | 0.33 | |||||
| OC use, n (%) | |||||||||
| Yes | 25 (55.6) | 159 (62.9) | 44 (62.0) | 142 (61.7) | 111 (63.4) | 75 (59.5) | 43 (62.3) | 74 (59.7) | 69 (63.9) |
| No | 20 (44.4) | 94 (37.2) | 27 (38.0) | 88 (38.3) | 64 (36.6) | 51 (40.5) | 26 (37.7) | 50 (40.3) | 39 (36.1) |
| p | 0.35 | 0.97 | 0.49 | 0.80 | |||||
| Alcohol consumption, g, median (IQR) | 2.1 (2.4) | 1.6 (4.7) | 2.1 (2.7) | 1.6 (4.1) | 1.7 (3.5) | 1.6 (5.0) | 2.1 (2.7) | 1.6 (5.1) | 1.5 (3.1) |
| p | 0.88 | 0.18 | 0.39 | 0.15 | |||||
| Smoking, n (%) | |||||||||
| Never | 14 (30.4) | 92 (35.1) | 24 (32.9) | 84 (35.3) | 65 (36.1) | 43 (32.8) | 24 (33.8) | 43 (33.3) | 41 (36.9) |
| Ever | 32 (69.6) | 170 (64.9) | 49 (67.1) | 154 (64.7) | 115 (63.9) | 88 (67.2) | 47 (66.2) | 86 (66.7) | 70 (63.1) |
| p | 0.54 | 0.70 | 0.55 | 0.83 | |||||
| Mother with BC, n (%) | |||||||||
| Yes | 1 (2.2) | 13 (5.3) | 5 (7.1) | 9 (4.0) | 9 (5.3) | 5 (4.0) | 5 (7.4) | 5. (4.1) | 4 (3.8) |
| No | 44 (97.8) | 234 (94.7) | 65 (92.9) | 216 (96.0) | 162 (94.7) | 119 (96.0) | 63 (92.7) | 117 (95.9) | 101 (96.2) |
| p | 0.38 | 0.28 | 0.62 | 0.51 | |||||
| Sister with BC, n (%) | |||||||||
| Yes | 1 (2.4) | 4 (1.8) | 1 (1.6) | 4 (2.0) | 4 (2.6) | 1 (0.9) | 1 (1.7) | 1 (0.9) | 3 (3.2) |
| No | 40 (97.6) | 218 (98.2) | 60 (98.4) | 201 (98.1) | 148 (97.4) | 113 (99.1) | 58 (98.3) | 111 (99.1) | 92 (96.8) |
| p | 0.78 | 0.88 | 0.30 | 0.49 | |||||
Associations between breast cancer risk factors and miR-20a-5p expression in tumor stromal fibroblasts (stroma), cancer cell cytoplasm (cytoplasm), cancer cell nuclei (nucleus), and cancer cell compartments combined. BC breast cancer, BMI body mass index, g grams, IQR interquartile range, OC oral contraceptive, SD standard deviation, y years. *Statistically significant (p < 0.05). **Menopausal status at diagnosis.