FIG. 5.

Increased IL-12 secretion by GMDC activates T-cell priming and CTL response. (a) Increased IL-12 production by GMDC. DC (left panel) and GMDC (right panel) were stained with antibodies, analyzed by flow cytometry, and plotted as IL-12 (20C2; PharMingen) versus Class II (Immu-375; Immunotech). (b) IL-12 augments Th1-type primary immune responses. Naïve peripheral lymphocytes (TC) were primed with GMDC (1:10 ratio) in the presence (right) and absence (left) of 5 ng of IL-12 (R&D) per ml. Seven days later, HIV-1-specific CTL were tested against autologous target DC (17) pulsed with hen egg lysozyme (■) or with hi-HIV-1 (⧫). The panels on the right of the graphs show the percentages of IFN-γ-producing CD8⁺ T cells after 3 days of priming with GMDC in the presence (right) and absence (left) of IL-12.