FIG. 1.

Myoblast differentiation increases transduction with rAAV-2cap5 but not rAAV-2 virus. Transduction of undifferentiated (A to D) and differentiated (E to H) C2C12 cells was evaluated for EGFP transgene expression following infection with 3,000 DNA particles/cell of either rAAV-2 (A, B, E, and F) or rAAV-2cap5 (C, D, G and H) virus for 24 h. EGFP expression was evaluated 72 h after infection by fluorescence microscopy. Nomarski and fluorescence photomicrographs are presented to the left and right of each panel, respectively. (I) Quantitative analysis of the percentage of EGFP-expressing cells. Values represent the means ± standard errors of the means (error bars) for more than 15 quantitated 10× magnification microscropic fields from three independent experiments.