FIG. 2.

Quantitative analysis of RSV-luciferase expression from rAAV-2 and rAAV-2cap5 virus in differentiated and undifferentiated C2C12 cells. (A) Undifferentiated and differentiated C2C12 cells were infected with either rAAV-2 or rAAV-2cap5 virus for 24 h at an MOI of 3,000 DNA particles/cell. Mock-infected cells were used as a negative control for background enzyme activity. The luciferase activity was determined at 24, 48, and 72 h after infection. (B) Ratio of relative luciferase expression (rAAV-2cap5/rAAV-2) for the two vector types. Values in both panels represent the means ± standard errors of the means (error bars) for three independent data points.