FIG. 4.

Proteasome inhibitors differentially affect rAAV-2 and rAAV-2cap5 transduction in differentiated C2C12 cells. To analyze the effect of proteasome inhibitors on the intracellular processing of different AAV serotypes, fully differentiated C2C12 cells were infected with either rAAV-2 or rAAV-2cap5 luciferase vectors at an MOI of 600 DNA particles/cell for 4 h. Tripeptide proteasome inhibitors (40 μM LLnL or 4 μM Z-LLL) were also added to the media during the infection period. Luciferase expression was quantified at 24 h postinfection. The data represent the means ± standard errors of the means (error bars) for three independent samples for each experimental condition.