Fig. 3. Proteolytic processing of Lingo2.

A Immunoblot analysis of the cell lysate and medium of the Lingo2 overexpressed HEK293A cells treated with or without metalloprotease inhibitor, GM6001. The soluble form of Ligno2 was diminished by the treatment of GM6001. B Immunoblot analysis of the cell lysate and medium of the Lingo2 overexpressed mouse primary neuron treated with or without metalloprotease inhibitory. C Quantification of sLingo2 protein level in mouse primary neurons. (n = 5, Mean ± SEM, One-way ANOVA was performed to assess the overall differences among groups. Post-hoc comparisons were conducted using Tukey’s HST test). The soluble form of Ligno2 was diminished by the administration of GM6001, INCB,3619, and GI254023X. D Immunoblot analysis of the cell lysate and medium of the Lingo2 overexpressed and ADAM family knockdown mouse primary neurons. E Quantification of sLingo2 protein level in mouse primary neurons. (n = 5, Mean ± SEM, One-way ANOVA was performed to assess the overall differences among groups. Post-hoc comparisons were conducted using Tukey’s HST test, *p < 0.05). The soluble form of Lingo2 was diminished by knockdown of the ADAM10. F Schematic representation of Lingo2 and its processing. G Locations of deletion mutants at the stalk region of Lingo2. H Immunoblot analysis of the cell lysate and medium of the mutant Lingo2-expressing HEK293 cells. Note that no expression of Δ534-545 mutant was observed. I Immunoblot analysis of the cell lysate and medium of the mutant Lingo2-expressing HEK293 cells.