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Published in final edited form as: Brain Behav Immun. 2023 Dec 22;116:269–285. doi: 10.1016/j.bbi.2023.12.025

Fig. 3. — Conditional ablation of microglial TNFR2 reduces the protein levels of pro-inflammatory cytokines after ischemic stroke. (A) Quantification of TNFR2 and TNFR1 protein levels in the brain of female Cx3cr1^CreER:Tnfrsf1b^fl/fl mice and Tnfrsf1b^fl/fl littermates in naïve conditions and at day 1 and 5 post-pMCAO (n = 5–12/group). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; Two-way ANOVA (TNFR2: Interaction^ns, Time**, Genotype****; TNFR1: Interaction^ns, Time****, Genotype^ns) followed by Bonferroni’s multiple comparisons test. (B) Quantification of TNF, IL-1β, IL-6, CXCL1, and CCL-5 protein levels in the brain of female Cx3cr1^CreER:Tnfrsf1b^fl/fl mice and Tnfrsf1b^fl/fl littermates in naïve conditions and at day 1 and 5 post-pMCAO (n = 5–12/group). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; Two-way ANOVA (TNF: Interaction****, Time****, Genotype****; IL-1β: Interaction****, Time****, Genotype***; IL-6: Interaction****, Time****, Genotype****; CXCL1: Interaction**, Time***, Genotype*; CCL-5: Interaction*, Time***, Genotype*) followed by Bonferroni’s multiple comparisons test. (C) Scatterplot representation of the linear correlation between TNFR2 (x axis) and TNF, IL-1β, IL-6, CXCL1, CCL-5 protein levels (y axis) in the brain of female Cx3cr1^CreER:Tnfrsf1b^fl/fl mice and Tnfrsf1b^fl/fl littermates at day 1 post-pMCAO (n = 4–5/group). For correlation analysis, two-tailed Pearson test was used. (D) Quantification of TNFR2 and TNFR1 protein levels in the brain of male Cx3cr1^CreER:Tnfrsf1b^fl/fl mice and Tnfrsf1b^fl/fl littermates in naïve conditions and at day 1 and 5 post-pMCAO (n = 5–11/group). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; Two-way ANOVA (TNFR2: Interaction^ns, Time***, Genotype****; TNFR1: Interaction^ns, Time****, Genotype^ns) followed by Bonferroni’s multiple comparisons test. (E) Quantification of TNF, IL-1β, IL-6, CXCL1, and CCL-5 protein levels in the brain of male Cx3cr1^CreER:Tnfrsf1b^fl/fl mice and Tnfrsf1b^fl/fl littermates in naïve conditions and at day 1 and 5 post-pMCAO (n = 5–11/group). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; Two-way ANOVA (TNF: Interaction^ns, Time****, Genotype^ns; IL-1β: Interaction^ns, Time****, Genotype*; IL-6: Interaction^ns, Time****, Genotype^ns; CXCL1: Interaction^ns, Time****, Genotype^ns; CCL-5: Interaction^ns, Time***, Genotype^ns) followed by Bonferroni’s multiple comparisons test. (F) Scatterplot representation of the linear correlation between TNFR2 (x axis) and TNF, IL-1β, IL-6, CXCL1, CCL-5 protein levels (y axis) in the brain of male Cx3cr1^CreER:Tnfrsf1b^fl/fl mice and Tnfrsf1b^fl/fl littermates at day 1 post-pMCAO (n = 5). For correlation analysis, two-tailed Pearson test was used.